Anti-Human SCF Rabbit Recombinant Antibody, PBS Only

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• Anti-Human SCF Rabbit Recombinant Antibody, PBS Only (98592-3-PBS)

SCF Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98592-3-PBS

Clone No.250446E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl

• 

  FC experiment of Human Bone marrow mesenchymal stromal cells using 98592-3-RR (same clone as 98592-3-PBS)

  1x10^6 human bone marrow mesenchymal stromal cells were intracellularly stained with 0.25 ug Anti-Human SCF Rabbit RecAb (98592-3-RR, Clone: 250446E11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98592-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Human Bone marrow mesenchymal stromal cells using 98592-3-RR (same clone as 98592-3-PBS)

  1x10^6 human bone marrow mesenchymal stromal cells were intracellularly stained with 0.25 ug Anti-Human SCF Rabbit RecAb (98592-3-RR, Clone: 250446E11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98592-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

98592-3-PBS targets SCF in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human SCF protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: KIT ligand
• Gene Symbol: SCF
• Gene ID (NCBI): 4254
• RRID: AB_3746201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21583
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit (PMID: 1698553, PMID: 8630386, PMID: 23073628). SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration (PMID: 28629522). SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells (PMID: 36688460). More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis (PMID: 18200049, PMID: 30157426).