SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13375-1-AP

Synonyms

Syntaxin-binding protein 1-like 1, STXBP1L1, Sec1 family domain-containing protein 2

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Validation Data Gallery

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  WB analysis of COLO 320 using 13375-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13375-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13375-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 13375-1-AP

  IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:200) with COLO 320 cells lysate 3200ug.

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  IHC staining of human colon cancer using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 13375-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse kidney tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCFD2 fusion protein Ag4197
• Full Name: sec1 family domain containing 2
• Calculated Molecular Weight: 684 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032453
• Gene Symbol: SCFD2
• Gene ID (NCBI): 152579
• RRID: AB_10646473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.