SCGB3A1 Polyclonal antibody

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• SCGB3A1 Polyclonal antibody (34191-1-AP)

SCGB3A1 Polyclonal Antibody for IHC, ELISA

Cat No. 34191-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

HIN1, Cytokine HIN-1, High in normal 1, Pneumo secretory protein 2, PNSP2

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  IHC staining of human lung using 34191-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 34191-1-AP (SCGB3A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 34191-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 34191-1-AP (SCGB3A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

34191-1-AP targets SCGB3A1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag39031

  Product name: Recombinant human SCGB3A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 21-104 aa of BC029176

  Sequence: FLVGSAKPVAQPVAALESAAEAGAGTLANPLGTLNPLKLLLSSLGIPVNHLIEGSQKCVAELGPQAVGAVKALKALLGALTVFG

• Full Name: secretoglobin, family 3A, member 1
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: BC029176
• Gene Symbol: SCGB3A1
• Gene ID (NCBI): 92304
• RRID: AB_3743169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96QR1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCGB3A1, also known as HIN-1 or UGRP2, belongs to the secretoglobin superfamily. This ~10 kDa secreted protein exhibits tumor suppressor activity through growth inhibition and is frequently silenced via promoter hypermethylation in breast, lung, and prostate cancers.( PMID: 30768367; 40691840)