SCGN Polyclonal antibody

SCGN Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14919-1-AP

Synonyms

CALBL, SCGN, SECRET, Secretagogin, SEGN, setagin

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Validation Data Gallery

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  WB analysis of mouse pancreas using 14919-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14919-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 14919-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14919-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 14919-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14919-1-AP

  IP result of anti-SCGN (IP:14919-1-AP, 4ug; Detection:14919-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, fetal human brain tissue, mouse colon tissue, mouse liver tissue, mouse stomach tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14919-1-AP targets SCGN in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCGN fusion protein Ag6736
• Full Name: secretagogin, EF-hand calcium binding protein
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000336
• Gene Symbol: SCGN
• Gene ID (NCBI): 10590
• RRID: AB_2183555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.