SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15093-1-AP

Synonyms

Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792

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Validation Data Gallery

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  WB analysis using 15093-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15093-1-AP

  Immunofluorescent analysis of HepG2 cells using 15093-1-AP (SCML1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15093-1-AP targets SCML1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCML1 fusion protein Ag5107
• Full Name: sex comb on midleg-like 1 (Drosophila)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 23 kDa，34kDa
• GenBank Accession Number: BC026159
• Gene Symbol: SCML1
• Gene ID (NCBI): 6322
• RRID: AB_2153243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.