SCN7A-Specific Polyclonal antibody

SCN7A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20256-1-AP

Synonyms

SCN6A, SCN7A, SCN7A-Specific

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Validation Data Gallery

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  WB analysis using 20256-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20256-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20256-1-AP targets SCN7A-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sodium channel, voltage-gated, type VII, alpha
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193 kDa
• GenBank Accession Number: NM_002976
• Gene Symbol: SCN7A
• Gene ID (NCBI): 6332
• RRID: AB_2878660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCN7A, also named as SCN6A, belongs to the sodium channel family. SCN7A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN7A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This antibody is specific to SCN7A.