Product Information
28707-1-PBS targets SCNN1A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30063 Product name: Recombinant human SCNN1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 440-604 aa of BC006526 Sequence: GSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSS Predict reactive species |
| Full Name | sodium channel, nonvoltage-gated 1 alpha |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC006526 |
| Gene Symbol | SCNN1A |
| Gene ID (NCBI) | 6337 |
| RRID | AB_2918193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37088 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Amiloride-sensitive sodium channel subunit alpha (SCNN1A, also known as Alpha-ENaC and ENaCA) is essential for maintaining sodium homeostasis and is particularly important in the distal nephron and late distal convoluted tubule of the kidney, where aldosterone triggers ENaC activity (PMID: 18086683; 31654198). The ~97 kDa and ~75kDa bands represent the glycosylated and unglycosylated forms of SCNN1A (PMID: 19462466). It also migrated as intact (≈ 75 kDa) and furin-cleaved (≈ 50 kDa) in urinary extracellular vesicles (uEVs) (PMID: 31654198).
