SCP2/SCPx Polyclonal antibody

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• KD/KO Validated

SCP2/SCPx Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14397-1-AP

Synonyms

SCP2, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16

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Validation Data Gallery

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  WB analysis using 14397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14397-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14397-1-AP

  WB result of SCP2/SCPx antibody (14397-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2/SCPx transfected HEK-293 cells.

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  IHC staining of human stomach using 14397-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14397-1-AP (SCP2/SCPx antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 14397-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14397-1-AP (SCP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1500
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14397-1-AP targets SCP2/SCPx in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCP2/SCPx fusion protein Ag5895
• Full Name: sterol carrier protein 2
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 44-46 kDa, 58 kDa
• GenBank Accession Number: BC067108
• Gene Symbol: SCP2
• Gene ID (NCBI): 6342
• RRID: AB_10644332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 14397-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase (PMID: 35996156).

Publications

Species	Application	Title
rat	WB	Redox Biol; The mammalian peroxisomal membrane is permeable to both GSH and GSSG - Implications for intraperoxisomal redox homeostasis; Authors - Maria J Ferreira
mouse	WB	EMBO Rep; MAPL loss dysregulates bile and liver metabolism in mice; Authors - Vanessa Goyon