SCP2/SCPx Polyclonal antibody

SCP2/SCPx Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19182-1-AP

Synonyms

NLTP, NSL TP, SCP 2, SCP chi, SCP X, SCP2, sterol carrier protein 2, Sterol carrier protein X

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Validation Data Gallery

• 

  WB analysis of A2780 using 19182-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19182-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19182-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Published Applications

• WB: See 4 publications below

Product Information

19182-1-AP targets SCP2/SCPx in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCP2/SCPx fusion protein Ag6002
• Full Name: sterol carrier protein 2
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 46 kDa, 58 kDa
• GenBank Accession Number: BC067108
• Gene Symbol: SCP2
• Gene ID (NCBI): 6342
• RRID: AB_10603471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 19182-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase.

Publications

Species	Application	Title
	WB	J Biol Chem; A Cargo-centered Perspective on the PEX5 Receptor-mediated Peroxisomal Protein Import Pathway.; Authors - Francisco Tania T
	WB	FEBS J; Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes.; Authors - Aurora Barros-Barbosa
rat	WB	J Biol Chem; The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo.; Authors - Ana F Dias
	WB	Methods Mol Biol; Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.; Authors - Tânia Francisco