SCPEP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis of U-251 using 83786-4-RR (same clone as 83786-4-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83786-4-RR (same clone as 83786-4-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83786-4-RR (same clone as 83786-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83786-4-RR (SCPEP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83786-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83786-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83786-4-RR against Human SCPEP1 were performed. The affinity constant is below 1 pM.

SCPEP1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83786-4-PBS

Clone No.240886B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-

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Product Information

83786-4-PBS targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag31381

  Product name: Recombinant human SCPEP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 221-452 aa of BC072405

  Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

• Full Name: serine carboxypeptidase 1
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 33 kDa, 45~51 kDa
• GenBank Accession Number: BC072405
• Gene Symbol: SCPEP1
• Gene ID (NCBI): 59342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9HB40
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).