SCRN1 Polyclonal antibody

SCRN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14303-1-AP

Synonyms

SES1, Secernin-1, secernin 1, KIAA0193

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Validation Data Gallery

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  WB analysis of A549 using 14303-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14303-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14303-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14303-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14303-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 14303-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14303-1-AP (SCRN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

14303-1-AP targets SCRN1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCRN1 fusion protein Ag5413
• Full Name: secernin 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC040492
• Gene Symbol: SCRN1
• Gene ID (NCBI): 9805
• RRID: AB_2878043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Publications

Species	Application	Title
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
mouse	WB	Biochem Biophys Res Commun; Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease.; Authors - Seong-Hun Chang
human	CoIP	J Mol Cell Biol; ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis; Authors - Xueyao Ma