SCRN2 Polyclonal antibody

SCRN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11485-2-AP

Synonyms

SCRN2, secernin 2, Ses2

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Validation Data Gallery

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  WB analysis of BxPC-3 using 11485-2-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11485-2-AP (SCRN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11485-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11485-2-AP (SCRN2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11485-2-AP targets SCRN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCRN2 fusion protein Ag2001
• Full Name: secernin 2
• Calculated Molecular Weight: 46.6 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC017317
• Gene Symbol: SCRN2
• Gene ID (NCBI): 90507
• RRID: AB_2184813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.