SCRN3 Polyclonal antibody

SCRN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 13240-1-AP

Synonyms

SES3, Secernin-3, secernin 3

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Validation Data Gallery

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  WB analysis of HepG2 using 13240-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13240-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13240-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13240-1-AP

  IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

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  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13240-1-AP

  Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13240-1-AP targets SCRN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCRN3 fusion protein Ag3777
• Full Name: secernin 3
• Calculated Molecular Weight: 424 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC031821
• Gene Symbol: SCRN3
• Gene ID (NCBI): 79634
• RRID: AB_2184816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kwan Ho (Verified Customer) (05-13-2022)

This Ab can detect endogenous SCRN3 in HEK293T cells, which was validated with KO cells. However, it also binds to off-targets.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells