SCRT1 Polyclonal antibody

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  WB analysis using 31577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31577-1-AP (SCRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SCRT1 Polyclonal Antibody for WB, ELISA

Cat No. 31577-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

SCRT, Scratch 1, hScrt

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Tested Applications

• Positive WB detected in: TT cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31577-1-AP targets SCRT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36215

  Product name: Recombinant human SCRT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-195 aa of BC168377

  Sequence: MPRSFLVKKVKLDAFSSADLESAYGRARSDLGAPLHDKGYLSDYVGPSSVYDGDAEAALLKGPSPEPMYAAAVRGELGPAAAGSAPPPTPRPELATAAGGYINGDAAVSEGYAADAFFITDGRSRRKASNAGAAAAPSTASAAAPDGDAGGGGGAGGRSLGSGPGGRGGTRAGAGTEARAGPGAAGAGGRHACGE

• Full Name: scratch homolog 1, zinc finger protein (Drosophila)
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC168377
• Gene Symbol: SCRT1
• Gene ID (NCBI): 83482
• RRID: AB_3670042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BWW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRT1 (Transcriptional repressor scratch 1), which is expressed in many tissues, especially in brain and adrenal gland. In addition, SCRT1 is also expressed in islet β cells, and its expression is related to insulin secretion ability. SCRT1 is a C2H2 zinc finger transcription inhibitor, which can bind to E-box motif. The encoded protein may promote nerve differentiation and may be related to cancer with neuroendocrine characteristics. The functions of SCRT1 in islet β cells include regulating the expression and secretion of insulin and influencing the proliferation of β cells. SCRT1 may control calcineurin /NFAT signaling pathway by affecting the expression of Syt4, Notch1, Nfatc1 and Nfatc2 genes, which is an important regulator of the growth and function of β cells (PMID: 33309639). The molecular weight of SCRT1 is 36 kDa.