SCYL3 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SCYL3 Polyclonal antibody (11249-1-AP)

• Featured Product
• KD/KO Validated

SCYL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11249-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCY1 like protein 3, Protein-associating with the carboxyl-terminal domain of ezrin, PACE1, PACE 1, Ezrin-binding protein PACE-1

• 

  WB analysis of human brain using 11249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11249-1-AP (SCYL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11249-1-AP (SCYL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SCYL3 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11249-1-AP targets SCYL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1769

  Product name: Recombinant human SCYL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-688 aa of BC014662

  Sequence: IPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVNIQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSAEQKPIPALLSLTEESMPWKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIKPSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW

• Full Name: SCY1-like 3 (S. cerevisiae)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC014662
• Gene Symbol: SCYL3
• Gene ID (NCBI): 57147
• RRID: AB_2071154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZE3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SCYL3 Polyclonal antibody (Cat# 11249-1-AP) has 1 citation. It appears in Journal of Neuroscience (IF 4.3), published in 2018. The cited study investigated the overlapping roles of SCYL1 and SCYL3 in maintaining motor neuron viability in mice, using WB and IF applications. The research field is neuroscience, specifically motor neuron biology and neurodegeneration.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	J Neurosci; Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability.; Authors - Emin Kuliyev ; KO Validated