SCYL3 Polyclonal antibody

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  WB analysis of human brain using 11249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11249-1-AP (SCYL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11249-1-AP (SCYL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SCYL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11249-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCY1 like protein 3, Protein-associating with the carboxyl-terminal domain of ezrin, PACE1, PACE 1, Ezrin-binding protein PACE-1

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11249-1-AP targets SCYL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1769

  Product name: Recombinant human SCYL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-688 aa of BC014662

  Sequence: IPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVNIQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSAEQKPIPALLSLTEESMPWKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIKPSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW

• Full Name: SCY1-like 3 (S. cerevisiae)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC014662
• Gene Symbol: SCYL3
• Gene ID (NCBI): 57147
• RRID: AB_2071154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZE3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	J Neurosci; Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability.; Authors - Emin Kuliyev ; KO Validated