SCYL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SCYL3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11249-1-AP

Synonyms

Ezrin binding protein PACE 1, PACE 1, PACE1, RP1 97P20.2, SCY1 like 3 (S. cerevisiae), SCY1 like protein 3, SCYL3

---

Validation Data Gallery

• 

  WB analysis of human brain using 11249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11249-1-AP (SCYL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11249-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11249-1-AP (SCYL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11249-1-AP targets SCYL3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCYL3 fusion protein Ag1769
• Full Name: SCY1-like 3 (S. cerevisiae)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC014662
• Gene Symbol: SCYL3
• Gene ID (NCBI): 57147
• RRID: AB_2071154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	J Neurosci; Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability.; Authors - Emin Kuliyev ; KO Validated