CD138/Syndecan-1 Monoclonal antibody

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  WB analysis of BxPC-3 using 60185-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60185-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

CD138/Syndecan-1 Monoclonal Antibody for WB, ELISA

Cat No. 60185-1-Ig

Clone No.1B6A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

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Tested Applications

• Positive WB detected in: BXPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60185-1-Ig targets CD138/Syndecan-1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0823

  Product name: Recombinant human SDC1,CD138 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC008765

  Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: CD138
• Gene ID (NCBI): 6382
• RRID: AB_10859833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P18827
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.