CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4H5H5

Cat no : 60185-2-Ig

Synonyms

CD138, SDC, SDC1, SYND1, syndecan, syndecan 1



Tested Applications

Positive WB detected inMCF7 cells
Positive IHC detected inhuman tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60185-2-Ig is CD138/Syndecan-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC008765
Gene symbol SDC1
Gene ID (NCBI) 6382
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

Protocols

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 60185-2-IgDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 60185-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols