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SDC4 Polyclonal antibody, PBS Only (11820-1-PBS)

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WB analysis using 11820-1-AP (same clone as 11820-1-PBS)
NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 11820-1-AP (SDC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SDC4 Polyclonal antibody, PBS Only

Name: SDC4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11820-1-PBS

SDC4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11820-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Amphiglycan, Ryudocan core protein, SYND4, syndecan 4, Syndecan-4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11820-1-PBS targets SDC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2388 Product name: Recombinant human SDC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC030805 Sequence: MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA Predict reactive species
Full Name	syndecan 4
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	22-26 kDa
GenBank Accession Number	BC030805
Gene Symbol	SDC4
Gene ID (NCBI)	6385
RRID	AB_2877797
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31431
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SDC4, also known as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4 may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa, and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer), which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )