SDCCAG8 Monoclonal antibody, PBS Only

SDCCAG8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1A4

Cat no : 66284-1-PBS

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

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Validation Data Gallery

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  WB analysis of HEK-293 using 66284-1-Ig (same clone as 66284-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66284-1-Ig (same clone as 66284-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66284-1-Ig (same clone as 66284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66284-1-Ig (same clone as 66284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66284-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66284-1-PBS targets SDCCAG8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDCCAG8 fusion protein Ag4264
• Full Name: serologically defined colon cancer antigen 8
• Calculated Molecular Weight: 713 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032454
• Gene Symbol: SDCCAG8
• Gene ID (NCBI): 10806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.