SDF4 Polyclonal antibody

SDF4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10517-1-AP

Synonyms

45 kDa calcium binding protein, Cab45, SDF 4, SDF4, stromal cell derived factor 4

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Validation Data Gallery

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  IHC staining of mouse testis using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10517-1-AP (SDF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10517-1-AP (SDF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10517-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse testis tissue, human pancreas tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

10517-1-AP targets SDF4 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDF4 fusion protein Ag0806
• Full Name: stromal cell derived factor 4
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC006211
• Gene Symbol: SDF4
• Gene ID (NCBI): 51150
• RRID: AB_2301345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IF, CoIP, IHC	Cell Death Discov; Fibroblast CEBPD/SDF4 axis in response to chemotherapy-induced angiogenesis through CXCR4.; Authors - Jhih-Ying Chi