SDHC Polyclonal antibody

SDHC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14575-1-AP

Synonyms

CYB560, CYBL, PGL3, QPs 1, QPS1, SDH3, SDHC

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Validation Data Gallery

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  WB analysis using 14575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14575-1-AP (SDHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14575-1-AP (SDHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14575-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14575-1-AP (SDHC antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 14575-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14575-1-AP (SDHC antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

14575-1-AP targets SDHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDHC fusion protein Ag5775
• Full Name: succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC033626
• Gene Symbol: SDHC
• Gene ID (NCBI): 6391
• RRID: AB_2183291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDHC(Succinate dehydrogenase cytochrome b560 subunit, mitochondrial) is also named as CYB560, SDH3 and belongs to the cytochrome b560 family. It is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3) and paraganglioma and gastric stromal sarcoma (PGGSS). It has a transit peptide of 29 amino acid and has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Nucleic Acids Res; Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice.; Authors - Johanna H K Kauppila
mouse	WB	iScience; Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver.; Authors - Tatsuya Yamada
mouse	WB	PLoS One; Quantitative proteomics in A30P*A53T α-synuclein transgenic mice reveals upregulation of Sel1l.; Authors - Jianguo Yan
human	WB	Cell Rep; Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress; Authors - Ying Liu
human	IHC	Front Immunol; Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma; Authors - Han Nan