SDSL Polyclonal antibody

SDSL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16242-1-AP

Synonyms

L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19

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Validation Data Gallery

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  WB analysis of HepG2 using 16242-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16242-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16242-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 16242-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16242-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16242-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16242-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16242-1-AP (SDSL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDSL fusion protein Ag9114
• Full Name: serine dehydratase-like
• Calculated Molecular Weight: 329 aa, 35 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC009849
• Gene Symbol: SDSL
• Gene ID (NCBI): 113675
• RRID: AB_2254163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.