SEC11C Polyclonal antibody

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  WB analysis using 16901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16901-1-AP (SEC11C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SEC11C Polyclonal Antibody for WB, ELISA

Cat No. 16901-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.4.21.89, SEC11 homolog C, SEC11 like protein 3, SEC11L3, SEC11-like protein 3

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16901-1-AP targets SEC11C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC11C fusion protein Ag10442
• Full Name: SEC11 homolog C (S. cerevisiae)
• Calculated Molecular Weight: 192 aa, 22 kDa
• Observed Molecular Weight: 16-22 kDa
• GenBank Accession Number: BC009703
• Gene Symbol: SEC11C
• Gene ID (NCBI): 90701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY50
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC11C (Signal peptidase complex catalytic subunit SEC11C), also known as SPC21. It is located in the Endoplasmic reticulum membrane. Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum 1. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids. The molecular weight of SEC11C is 22 kDa. It has been reported that its molecular weight is 16kDa (PMID: 38183983).