SEC24C Polyclonal antibody, PBS Only

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• SEC24C Polyclonal antibody, PBS Only (16073-1-PBS)

• 

  WB analysis of HeLa using 16073-1-AP (same clone as 16073-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16073-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16073-1-AP (same clone as 16073-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16073-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16073-1-AP (same clone as 16073-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16073-1-AP (SEC24C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16073-1-PBS in a different storage buffer formulation.

SEC24C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16073-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0079, Protein transport protein Sec24C, SEC24-related protein C

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Product Information

16073-1-PBS targets SEC24C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9030

  Product name: Recombinant human SEC24C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 742-1094 aa of BC018928

  Sequence: DVQKVVGFDAVMRVRTSTGIRAVDFFGAFYMSNTTDVELAGLDGDKTVTVEFKHDDRLNEESGALLQCALLYTSCAGQRRLRIHNLALNCCTQLADLYRNCETDTLINYMAKFAYRGVLNSPVKAVRDTLITQCAQILACYRKNCASPSSAGQLILPECMKLLPVYLNCVLKSDVLQPGAEVTTDDRAYVRQLVTSMDVTETNVFFYPRLLPLTKSPVESTTEPPAVRASEERLSNGDIYLLENGLNLFLWVGASVQQGVVQSLFSVSSFSQITSGLSVLPVLDNPLSKKVRGLIDSLRAQRSRYMKLTVVKQEDKMEMLFKHFLVEDKSLSGGASYVDFLCHMHKEIRQLLS

• Full Name: SEC24 family, member C (S. cerevisiae)
• Calculated Molecular Weight: 1094 aa, 118 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC018928
• Gene Symbol: SEC24C
• Gene ID (NCBI): 9632
• RRID: AB_2186121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53992
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).