Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells, U2OS cells, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24935-1-AP targets SEC61 Alpha in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20779 Product name: Recombinant human SEC61A2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 190-418 aa of BC026179 Sequence: TIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNSARA Predict reactive species |
| Full Name | Sec61 alpha 2 subunit (S. cerevisiae) |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC026179 |
| Gene Symbol | SEC61 Alpha |
| Gene ID (NCBI) | 55176 |
| RRID | AB_2879807 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9S3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC61 Alpha antibody 24935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
SEC61 Alpha Polyclonal antibody (24935-1-AP) has a total of 3 citations appearing in Advanced Science, Science Advances, and iScience. The associated research fields include lysosomal-ER calcium signaling at membrane contact sites, tumor immunology focusing on MHC-I degradation and impaired CD8+ T cell responses, and endoplasmic reticulum proteostasis of GABAA receptors in neuroscience.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium | ||
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
iScience The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors |
Reviews
What customers say
One reviewer (Boyan Zhang, Dec 2021) rated this antibody 1 star for Western Blot. They reported that while it recognized a band around 60 kDa as expected, it also produced a similar band when run with loading buffer alone, suggesting non-specific binding or contamination concerns. No other reviews are currently available for this product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (12-16-2021) | This antibody recognized a band around 60 kd, but it also recognized a similar band in solo loading buffer.
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