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SELENBP1 Polyclonal antibody, PBS Only (27449-1-PBS)

Filter: AllWBIHC

WB analysis using 27449-1-AP (same clone as 27449-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 27449-1-AP (same clone as 27449-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 27449-1-AP (same clone as 27449-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 27449-1-AP (same clone as 27449-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 27449-1-AP (same clone as 27449-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 27449-1-AP (same clone as 27449-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 27449-1-AP (same clone as 27449-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 27449-1-AP (same clone as 27449-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27449-1-PBS in a different storage buffer formulation.

SELENBP1 Polyclonal antibody, PBS Only

Name: SELENBP1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27449-1-PBS

SELENBP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27449-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27449-1-PBS targets SELENBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24453 Product name: Recombinant human SELENBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 121-472 aa of BC009084 Sequence: VIEPKDIHAKCELAFLHTSHCLASGEVMISSLGDVKGNGKGGFVLLDGETFEVKGTWERPGGAAPLGYDFWYQPRHNVMISTEWAAPNVLRDGFNPADVEAGLYGSHLYVWDWQRHEIVQTLSLKDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKNEGGTWSVEKVIQVPPKKVKGWLLPEMPGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLFLGGSIVKGGPVQVLEDEELKSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIREGSVMLQVDVDTVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI Predict reactive species
Full Name	selenium binding protein 1
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	56 kDa
GenBank Accession Number	BC009084
Gene Symbol	SELENBP1
Gene ID (NCBI)	8991
RRID	AB_2880872
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13228
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SELENBP1 (selenium binding protein 1,SBP1), also known as LPSB or SP56, is a 472 amino acid peripheral membrane protein that binds selenium covalently. Selenium, an essential trace element, has been shown to have an anti-cancer effect. Many reports have described a relationship between insufficient selenium intake and increased risk of cancer. Moreover, the expression of SELENBP1 has been shown to be decreased in several tumors including cancers of the prostate, lungs, colon, and ovary (PMID: 21143902, 20332323, 18272210). SELENBP1 has four isoforms with the molecular mass of 56kD, 52kD and 45kD.