Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, K-562 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32860-1-AP targets SELENOO in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35939 Product name: Recombinant human RP3-402G11.5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 477-666 aa of BC110866 Sequence: LARLMEQCASLEELRLAFRPQMDPRQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT Predict reactive species |
| Full Name | selenoprotein O |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC110866 |
| Gene Symbol | RP3-402G11.5 |
| Gene ID (NCBI) | 83642 |
| RRID | AB_3742767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9BVL4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SELENOO (Selenoprotein O) is a selenocysteine-containing (Sec) selenoprotein belonging to the oxidoreductase family, which is mainly localized in the endoplasmic reticulum (ER) membranes with multiple transmembrane structural domains. SELENOO plays important roles in a variety of cellular processes, including redox homeostasis and lipid metabolism and DNA damage repair. It plays an important role in a variety of cellular processes, including redox homeostasis, lipid metabolism, and DNA damage repair. SELENOO is aberrantly expressed in a variety of diseases, including cancer and neurodegenerative diseases. For example, in melanoma and lung cancer, the upregulation of SELENOO expression is associated with tumor progression and metastasis, which has become a hot topic in current research.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SELENOO antibody 32860-1-AP | Download protocol |
| WB protocol for SELENOO antibody 32860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SELENOO Polyclonal antibody (Cat# 32860-1-AP) has 1 total citation. It appears in Molecular Cell, a high-impact journal (IF: 16). The cited study investigates hypoxia-driven lncRNA SHIELD promoting GPX4 translation to protect against ferroptosis in hepatocellular carcinoma, placing this citation in the research fields of ferroptosis, oxidative stress regulation, and liver cancer biology.
| Species | Application | Title |
|---|---|---|
Mol Cell Hypoxia-driven lncRNA SHIELD promotes GPX4 translation and protects against ferroptosis in hepatocellular carcinoma. |
