SELK Polyclonal antibody

SELK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12561-1-AP

Synonyms

Selenoprotein K, HSPC297, HSPC030

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Validation Data Gallery

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  WB analysis of K-562 using 12561-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12561-1-AP (SELK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12561-1-AP targets SELK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SELK fusion protein Ag3143
• Full Name: selenoprotein K
• Calculated Molecular Weight: 94 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC013162
• Gene Symbol: SELK
• Gene ID (NCBI): 58515
• RRID: AB_2186964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Selenoprotein K (SelK), a single-pass transmembrane protein that resides in the endoplasmic reticulum (ER) membrane, which has been reported to an emerging role of playing a part in protein palmitoylation. Human SelK is a 94-amino-acid protein with Sec residing at the penultimate position. It has been shown that SelK is expressed predominantly in the heart and skeletal muscle, but other tissues, such as the liver, pancreas, and placenta, also have detectable levels of SelK.(PMID: 36252341)

Publications

Species	Application	Title
human,mouse	WB	Biochem Biophys Res Commun; Knockdown of Trnau1ap inhibits the proliferation and migration of NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway.; Authors - Xiaoyan Hu
mouse	WB	Front Pharmacol; Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.; Authors - Chang-lan Chen
rat	WB	Cell Mol Life Sci; Selenium-SelK-GPX4 axis protects nucleus pulposus cells against mechanical overloading-induced ferroptosis and attenuates senescence of intervertebral disc; Authors - Chunwang Jia