P-selectin / CD62P Polyclonal antibody

P-selectin / CD62P Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13304-1-AP

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP



Tested Applications

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13304-1-AP targets P-selectin / CD62P in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P-selectin / CD62P fusion protein Ag4124
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
GenBank accession numberBC028067
Gene symbol SELP
Gene ID (NCBI) 6403
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
WB protocol for P-selectin / CD62P antibody 13304-1-APDownload protocol
IHC protocol for P-selectin / CD62P antibody 13304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Appl Mater Interfaces

AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer.

Authors - Yanhong Duo
humanFC

World J Gastroenterol

Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

Authors - Jia-Cheng Li
humanIF,FC

Immun Inflamm Dis

Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.

Authors - Shihua Zhang