P-selectin / CD62P Polyclonal antibody

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  WB analysis of human peripheral blood platelets using 13304-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P-selectin / CD62P Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13304-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below
• FC: See 2 publications below

Product Information

13304-1-AP targets P-selectin / CD62P in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P-selectin / CD62P fusion protein Ag4124
• Full Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Calculated Molecular Weight: 830 aa, 91 kDa
• GenBank Accession Number: BC028067
• Gene Symbol: CD62P
• Gene ID (NCBI): 6403
• RRID: AB_2877937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16109
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Publications

Species	Application	Title
mouse	WB	ACS Appl Mater Interfaces; AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer.; Authors - Yanhong Duo
mouse	WB	ACS Appl Mater Interfaces; Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression; Authors - Yingke Liu
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
mouse	IF	iScience; Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis; Authors - Weijia Zhou
human	FC	World J Gastroenterol; Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer; Authors - Jia-Cheng Li
human	IF,FC	Immun Inflamm Dis; Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.; Authors - Shihua Zhang