P-selectin / CD62P Monoclonal antibody

P-selectin / CD62P Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2C7

Cat no : 60322-1-Ig

Synonyms

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP



Tested Applications

Positive WB detected inhuman peripheral blood platelets, Jurkat cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60322-1-Ig targets P-selectin / CD62P in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P-selectin / CD62P fusion protein Ag4124
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated molecular weight 830 aa, 91 kDa
Observed molecular weight 140 kDa, 105-110 kDa
GenBank accession numberBC028067
Gene symbol SELP
Gene ID (NCBI) 6403
RRIDAB_2881433
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
WB protocol for P-selectin / CD62P antibody 60322-1-IgDownload protocol
IP protocol for P-selectin / CD62P antibody 60322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Biomater

Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection.

Authors - Wenyan Yu
humanWB

Front Oncol

An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.

Authors - Hongwei Jin
mouseWB

Front Oncol

Platelet Membranes Coated Gold Nanocages for Tumor Targeted Drug Delivery and Amplificated Low-Dose Radiotherapy.

Authors - Mingzhu Chen
humanFC

Commun Biol

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.

Authors - LinXi Cheng
mouseWB

Front Bioeng Biotechnol

Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor.

Authors - Chunyu Huang
humanWB

Biol Res

CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis.

Authors - Xiaohao Zhang