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  • KD/KO Validated

SELPLG Polyclonal antibody

SELPLG Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23605-1-AP

Synonyms

CD162, CLA, PSGL 1, PSGL1, selectin P ligand, SELPLG



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

23605-1-AP targets SELPLG in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SELPLG fusion protein Ag19394
Full Name selectin P ligand
Calculated molecular weight 412 aa, 43 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC029782
Gene symbol SELPLG
Gene ID (NCBI) 6404
RRIDAB_2879302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SELPLG antibody 23605-1-APDownload protocol
IHC protocol for SELPLG antibody 23605-1-APDownload protocol
IF protocol for SELPLG antibody 23605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Theranostics

Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages

Authors - Xueqin Li
  • KD Validated
humanWB

J Neuroinflammation

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Authors - Ceshu Gao
humanIF

Front Cell Dev Biol

Molecular and Clinicopathological Characterization of a Prognostic Immune Gene Signature Associated With MGMT Methylation in Glioblastoma.

Authors - Liang Zhao
IF

Inflammation

Carbon Monoxide-Releasing Molecule-3 Regulates the Polarization of Lipopolysaccharide-Induced Macrophages.

Authors - Tingting Liu
humanWB

Adv Biol (Weinh)

Natural Oncolysis of Enterovirus 71 in Antitumor Therapy of Colorectal Cancer

Authors - Zhihui Ruan