SELPLG Polyclonal antibody

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  WB analysis of Jurkat using 23605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23605-1-AP (SELPLG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 23605-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23605-1-AP (SELPLG Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 23605-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23605-1-AP (SELPLG Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of Jurkat using 23605-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (23605-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SELPLG Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 23605-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

CD162, CLA, PSGL 1, PSGL1, selectin P ligand, SELPLG

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Product Information

23605-1-AP targets SELPLG in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SELPLG fusion protein Ag19394
• Full Name: selectin P ligand
• Calculated Molecular Weight: 412 aa, 43 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC029782
• Gene Symbol: SELPLG
• Gene ID (NCBI): 6404
• RRID: AB_2879302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14242
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li ; KD Validated
human	WB	J Neuroinflammation; Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.; Authors - Ceshu Gao
human	IF	Front Cell Dev Biol; Molecular and Clinicopathological Characterization of a Prognostic Immune Gene Signature Associated With MGMT Methylation in Glioblastoma.; Authors - Liang Zhao
	IF	Inflammation; Carbon Monoxide-Releasing Molecule-3 Regulates the Polarization of Lipopolysaccharide-Induced Macrophages.; Authors - Tingting Liu
human	WB	Adv Biol (Weinh); Natural Oncolysis of Enterovirus 71 in Antitumor Therapy of Colorectal Cancer; Authors - Zhihui Ruan