SELPLG/CD162 Recombinant monoclonal antibody, PBS Only

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  IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84500-1-RR (same clone as 84500-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (84500-1-RR, Clone: 241297G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84500-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84500-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.221 nM.

SELPLG/CD162 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 84500-1-PBS

Clone No.241297G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

SELPLG, 241297G10, CD162, CLA, P-selectin glycoprotein ligand 1

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Product Information

84500-1-PBS targets SELPLG/CD162 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: selectin P ligand
• Calculated Molecular Weight: 412 aa, 43 kDa
• GenBank Accession Number: BC029782
• Gene Symbol: SELPLG
• Gene ID (NCBI): 6404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14242
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).