SEMA4F Polyclonal antibody

SEMA4F Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12474-1-AP

Synonyms

M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW

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Validation Data Gallery

• 

  WB analysis of mouse lung using 12474-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12474-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12474-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12474-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12474-1-AP

  IP result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: mouse lung tissue, HepG2 cells, human liver tissue, mouse brain tissue, Y79 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12474-1-AP targets SEMA4F in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA4F fusion protein Ag3082
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
• Calculated Molecular Weight: 615 aa, 66 kDa
• Observed Molecular Weight: 66 kDa, 84 kDa
• GenBank Accession Number: BC018361
• Gene Symbol: SEMA4F
• Gene ID (NCBI): 10505
• RRID: AB_2185847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.