Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22571-1-AP targets SEMA6B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16707 Product name: Recombinant human SEMA6B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 625-888 aa of BC142617 Sequence: RERRELARRKDKEAILAHGAGEAVLSVSRLGERRAQGPGGRGGGGGGGAGVPPEALLAPLMQNGWAKATLLQGGPHDLDSGLLPTPEQTPLPQKRLPTPHPHPHALGPRAWDHGHPLLPASASSSLLLLAPARAPEQPPAPGEPTPDGRLYAARPGRASHGDFPLTPHASPDRRRVVSAPTGPLDPASAADGLPRPWSPPPTGSLRRPLGPHAPPAATLRRTHTFNSGEARPGDRHRGCHARPGTDLAHLLPYGGADRTAPPVP Predict reactive species |
| Full Name | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B |
| Calculated Molecular Weight | 95, 74 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC142617 |
| Gene Symbol | SEMA6B |
| Gene ID (NCBI) | 10501 |
| RRID | AB_3742073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9H3T3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Semaphorins (SEMAs) play critical roles in inflammation, angiogenesis, apoptosis, fibrosis, bone remodeling, and cell invasion. SEMA6B, the sixth member of the SEMA family, has shown significant correlations with the stage of liver fibrosis in hepatitis C virus-infected patients. SEMA6B is also involved in inflammatory responses and serves as a biomarker for macrophage-specific genes. Additionally, the level of SEMA6B in circulating monocytes has predictive value for cardiovascular disease. Also, SEMA6B can be a valuable biomarker for diagnosing HBV-ACLF12 (PMID: 39267780).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SEMA6B antibody 22571-1-AP | Download protocol |
| WB protocol for SEMA6B antibody 22571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SEMA6B Polyclonal antibody (Cat# 22571-1-AP) has 1 citation to date. The cited publication appeared in Molecular Cancer Research (Impact Factor 5.8) and investigated how USP7 inhibition destabilizes the noncanonical PRC1.1 complex and induces neuroblastoma differentiation. The antibody was used in Western Blot (WB) on human samples. The research field is oncology, specifically neuroblastoma biology and protein ubiquitination pathways.
| Species | Application | Title |
|---|---|---|
Mol Cancer Res Inhibition of USP7 destabilizes the noncanonical PRC1.1 complex and induces neuroblastoma differentiation. |
