SEMA6B Polyclonal antibody

• 

  WB analysis using 22571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22571-1-AP (SEMA6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22571-1-AP

  IP result of anti-SEMA6B (IP:22571-1-AP, 4ug; Detection:22571-1-AP 1:1000) with mouse brain tissue lysate 1120 ug.

SEMA6B Polyclonal Antibody for WB, ELISA

Cat No. 22571-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

SEM SEMA Y, SEMA VIB, Sema Z, SEMAN, Semaphorin 6B

---

Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22571-1-AP targets SEMA6B in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA6B fusion protein Ag16707
• Full Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
• Calculated Molecular Weight: 95, 74 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC142617
• Gene Symbol: SEMA6B
• Gene ID (NCBI): 10501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9H3T3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Semaphorins (SEMAs) play critical roles in inflammation, angiogenesis, apoptosis, fibrosis, bone remodeling, and cell invasion. SEMA6B, the sixth member of the SEMA family, has shown significant correlations with the stage of liver fibrosis in hepatitis C virus-infected patients. SEMA6B is also involved in inflammatory responses and serves as a biomarker for macrophage-specific genes. Additionally, the level of SEMA6B in circulating monocytes has predictive value for cardiovascular disease. Also, SEMA6B can be a valuable biomarker for diagnosing HBV-ACLF12 (PMID: 39267780).