Tested Applications
| Positive IHC detected in | mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15916-1-AP targets Semenogelin-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8717 Product name: Recombinant human SEMG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC007096 Sequence: MKPNIIFVLSLLLILEKQAAVMGQKGGSKGRLPSEFSQFPHGQKGQHYSGQKGKQQTESKGSFSIQYTYHVDANDHDQSRKSQQYDLNALHKTTKSQRHLGGSQQLLHNKQEGRDHDKSKGHFHRVVIHHKGGKAHRGTQNPSQDQGNSPSGKGISSQYSNTEERLWVHGLSKEQTSVSGAQKGRKQGGSQSSYVLQTEELVANKQQRETKNSHQNKGHYQNVVEVREEHSSKVQTSLCPAHQDKLQHGSKDIFSTQDELLVYNKNQHQTKNLNQDQQHGRKANKISYQSSSTEERRLHYGENGVQKDVSQR Predict reactive species |
| Full Name | semenogelin I |
| Calculated Molecular Weight | 402aa,45 kDa; 462aa,52 kDa |
| GenBank Accession Number | BC007096 |
| Gene Symbol | Semenogelin-1 |
| Gene ID (NCBI) | 6406 |
| RRID | AB_2270146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Semenogelin-1 antibody 15916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Semenogelin-1 Polyclonal antibody (Cat# 15916-1-AP) has a total of 1 citation. It appears in Biology of Reproduction (IF: 3.2), published in January 2022. The cited study investigated how blocking serine protease activity prevents Semenogelin degradation, leading to hyperviscous semen in humans. The associated research field is reproductive biology and male infertility. The antibody was used for Western blotting on human samples.
| Species | Application | Title |
|---|---|---|
Biol Reprod Blocking serine protease activity prevents Semenogelin degradation leading to Hyperviscous semen in humans. |
