SENP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83594-4-RR (same clone as 83594-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83594-4-RR (same clone as 83594-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83594-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

SENP1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83594-4-PBS

Clone No.240478D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

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Product Information

83594-4-PBS targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18028

  Product name: Recombinant human SENP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC045639

  Sequence: MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREVYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTP

• Full Name: SUMO1/sentrin specific peptidase 1
• Calculated Molecular Weight: 644 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC045639
• Gene Symbol: SENP1
• Gene ID (NCBI): 29843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9P0U3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.