KD/KO Validated

Septin 1 Polyclonal antibody

Septin 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11370-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

SEPT1, Peanut-like protein 3, SEPT 1, septin1, Septin-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of Jurkat using 11370-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11370-1-AP (Septin 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 11370-1-AP
WB result of Septin 1 antibody (11370-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 1 transfected Jurkat cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse thymus using 11370-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11370-1-AP (Septin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 11370-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11370-1-AP (Septin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 11370-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11370-1-AP (Septin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 11370-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11370-1-AP (Septin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat thymus using 11370-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11370-1-AP (Septin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Septin 1 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells
Positive IHC detected in	human tonsillitis tissue, rat spleen tissue, mouse thymus tissue, rat thymus tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11370-1-AP targets Septin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1926 Product name: Recombinant human SEPT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-367 aa of BC012161 Sequence: MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARCLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQQSQAQGEQSDAL Predict reactive species
Full Name	septin 1
Calculated Molecular Weight	367 aa, 42 kDa
Observed Molecular Weight	40-42 kDa
GenBank Accession Number	BC012161
Gene Symbol	Septin 1
Gene ID (NCBI)	1731
RRID	AB_2301671
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WYJ6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Septin 1 antibody 11370-1-AP	Download protocol
WB protocol for Septin 1 antibody 11370-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Septin 1 Polyclonal antibody (catalog number 11370-1-AP) has a total of 1 citation. This citation appears in the Journal of Cell Biology (J Cell Biol), published in July 2020, with an impact factor of 6.1. The study, titled "The septin cytoskeleton regulates natural killer cell lytic granule release," used the antibody for Western blot (WB) and immunofluorescence (IF) applications in human samples. The associated research field is cell biology, specifically focusing on the septin cytoskeleton and its role in natural killer cell lytic granule release.