Tested Applications
| Positive WB detected in | rat testis tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24590-1-AP targets Septin 14 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20181 Product name: Recombinant human SEPT14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 389-432 aa of BC153166 Sequence: IRKLEEEKKQLEGEIIDFYKMKAASEALQTQLSTDTKKDKHRKK Predict reactive species |
| Full Name | septin 14 |
| Calculated Molecular Weight | 432 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC153166 |
| Gene Symbol | Septin 14 |
| Gene ID (NCBI) | 346288 |
| RRID | AB_2879626 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZU15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 14 antibody 24590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Septin 14 (SEPT14) polyclonal antibody (Cat# 24590-1-AP) has a total of 3 citations. These appear in Cell Death & Disease, Ecotoxicology and Environmental Safety, and Biomedicines. The associated research fields include reproductive biology (sperm flagellum formation and head shaping), environmental toxicology (PFOA exposure effects on sperm), and genetic studies of SEPT14 mutation-induced sperm defects. Applications cited include WB and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||



