Septin 5 Polyclonal antibody

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• KD/KO Validated

Septin 5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11631-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SEPT5, CDCREL, CDCREL 1, CDCREL1, CDCrel-1

• 

  WB analysis of Jurkat using 11631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U2OS using 11631-1-AP

  WB result of Septin 5 antibody (11631-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 5 transfected U2OS cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 11631-1-AP

  Human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 11631-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 11631-1-AP

  IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat cerebellum using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, NIH/3T3 cells, mouse brain tissue, U2OS cells, rat brain tissue, K-562 cells, LNCaP cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse cerebellum tissue, human placenta tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11631-1-AP targets Septin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2205

  Product name: Recombinant human SEPT5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-369 aa of BC025261

  Sequence: MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ

• Full Name: septin 5
• Calculated Molecular Weight: 369 aa, 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC025261
• Gene Symbol: Septin 5
• Gene ID (NCBI): 5413
• RRID: AB_2301692
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99719
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Publications

What published studies show

The anti-SEPT5 antibody (Cat# 11631-1-AP) has 10 citations published in journals including Nature Communications, Journal of Advanced Research, Neurobiology of Disease, Cells, Molecules, Cancers, Cell Signaling, Frontiers in Molecular Neuroscience, Journal of Neuroscience, and Cytoskeleton. Research fields span β-cell exocytosis, thrombus formation, Alzheimer's pathology, APP/Aβ degradation, neuroprotection, endometrial cancer, renal cell carcinoma, hippocampal aging, exocytosis regulation, and kidney fibrosis.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat,mouse,human	WB,IF	Nat Commun; Septin5 deletion enhances β-cell exocytosis by releasing microtubule-tethered insulin granules onto plasma membrane; Authors - Li Xie
rat,mouse,human	WB	J Adv Res; Pharmacological inhibition of Septins with Forchlorfenuron attenuates thrombus formation in experimental thrombotic mice models with modulating multiple signaling pathways in platelets; Authors - Zhen Hao
mouse	WB	Neurobiol Dis; S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5.; Authors - Catarina B Ferreira
human	WB,IF	Cells; Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of Aβ.; Authors - Mikael Marttinen ; KO Validated
rat	WB	Molecules; Synergistic Neuroprotective Effect of Endogenously-Produced Hydroxytyrosol and Synaptic Vesicle Proteins on Pheochromocytoma Cell Line Against Salsolinol.; Authors - Robina Manzoor
human	WB	Cancers (Basel); Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer; Authors - Kyukwang Kim