Septin 7 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA, PLA

Conjugate

Unconjugated

Publications

28

Reviews (2)

Cat no : 13818-1-AP

Synonyms

septin7, SEPT7A, SEPT7, SEPT 7, CDC3

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Validation Data Gallery

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  WB analysis using 13818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13818-1-AP

  WB result of Septin 7 antibody (13818-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells.

• 

  WB analysis of mouse testis using 13818-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13818-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 13818-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE cells using 13818-1-AP

  IF result of anti-Septin 7 (13818-1-AP, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 7. Blue: DNA. 1% PFA fixed.

• 

  IF Staining of HepG2 using 13818-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IF: See 18 publications below
• CoIP: See 1 publications below

Product Information

13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 7 fusion protein Ag4907
• Full Name: septin 7
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC025987
• Gene Symbol: Septin 7
• Gene ID (NCBI): 989
• RRID: AB_2254298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Publications

Species	Application	Title
		Nat Commun; Still and rotating myosin clusters determine cytokinetic ring constriction.; Authors - Viktoria Wollrab
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar ; KD Validated
mouse	WB,IF	Cell Rep; Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.; Authors - Fernando Calvo
mouse	IF,PLA	EMBO Rep; Cdc42-Borg4-Septin7 axis regulates HSC polarity and function; Authors - Ravinder Kandi
human	WB,IF	MBio; Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.; Authors - Quynh T Phan ; KD Validated
bovine	IF	Development; Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling.; Authors - Mengyun Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amaury (Verified Customer) (03-29-2022)

I performed immunolabelling on K562. Used ice-cold methanol to permeabilize the cells and obtain very nice labelling

• Applications: Immunofluorescence
• Cell Tissue Type: K562

Septin 7 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:13818-1-AP)

FH

stephen (Verified Customer) (02-19-2019)

HeLa cells fixed with 4%PFA and permblised with 0.3% triton x100 for 5 mins. stained with septin 7ab 1:300. (green) and DAPI to mark DNA (blue). non dividing interphase cell (top). Dividing cell in anaphase (bottom)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa cells

Septin 7 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa cells (Cat no:13818-1-AP)

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