Septin 8 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11769-1-AP

Synonyms

SEPT8, Septin-8, SEPTIN8, SEP2, KIAA0202

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Validation Data Gallery

• 

  WB analysis of HeLa using 11769-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11769-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11769-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11769-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11769-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11769-1-AP

  IP result of anti-Septin 8 (IP:11769-1-AP, 3ug; Detection:11769-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (Septin 8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (SEPT8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 11769-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 8 antibody (11769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 5 publications below

Product Information

11769-1-AP targets Septin 8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 8 fusion protein Ag2357
• Full Name: septin 8
• Calculated Molecular Weight: 55.6 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC001329
• Gene Symbol: Septin 8
• Gene ID (NCBI): 23176
• RRID: AB_2185006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are 40-60kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. It may play a role in platelet secretion. Septin 8 is about 50-56 kDa in WB detection.

Publications

Species	Application	Title
mouse	WB,IF	Elife; Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.; Authors - Julia Patzig
mouse	WB,IF	Thromb Haemost; Impaired Platelet Function in Sept8-Deficient Mice In Vitro; Authors - Katharina Neubauer
mouse	WB	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems
		Glia; The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.; Authors - Andrew A Jarjour
mouse	WB	Development; Pinch2 regulates myelination in the mouse central nervous system.; Authors - Joana Paes de Faria
mouse, human	WB	J Cell Sci; SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.; Authors - Kaisa M A Kurkinen ; KD Validated