Septin 9 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 82844-2-RR (same clone as 82844-2-PBS)

  WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 82844-2-RR (same clone as 82844-2-PBS)

  WB result of Septin 9 antibody (82844-2-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 9 transfected HepG2 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 82844-2-RR (same clone as 82844-2-PBS)

  IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82844-2-RR (same clone as 82844-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82844-2-RR (same clone as 82844-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82844-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

Septin 9 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 82844-2-PBS

Clone No.2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A

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Product Information

82844-2-PBS targets Septin 9 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1218

  Product name: Recombinant human SEPT9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 250-568 aa of BC021192

  Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM

• Full Name: septin 9
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65-80 kDa, 38-40 kDa
• GenBank Accession Number: BC021192
• Gene Symbol: SEPT9
• Gene ID (NCBI): 10801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UHD8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).