SERAC1 Polyclonal antibody

SERAC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25729-1-AP

Synonyms

Protein SERAC1, SERAC1

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Validation Data Gallery

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  WB analysis of mouse brain using 25729-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25729-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 25729-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human colon using 25729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 25729-1-AP

  Immunofluorescent analysis of HepG2 cells using 25729-1-AP (SERAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25729-1-AP is SERAC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERAC1 fusion protein Ag22790
• Full Name: serine active site containing 1
• Calculated Molecular Weight: 654 aa, 74 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001705
• Gene Symbol: SERAC1
• Gene ID (NCBI): 84947
• RRID: AB_2880213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.