SERBP1 Polyclonal antibody

SERBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10729-1-AP

Synonyms

CGI 55, CHD3IP, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

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Validation Data Gallery

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  WB analysis using 10729-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10729-1-AP (SERBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10729-1-AP (SERBP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10729-1-AP (SERBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10729-1-AP (SERBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 10729-1-AP (SERBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10729-1-AP targets SERBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERBP1 fusion protein Ag1045
• Full Name: SERPINE1 mRNA binding protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008045
• Gene Symbol: SERBP1
• Gene ID (NCBI): 26135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

Publications

Species	Application	Title
		Cell Rep; Triggering endogenous Z-RNA sensing for anti-tumor therapy through ZBP1-dependent necroptosis; Authors - Tao Yang
human	WB	J Clin Endocrinol Metab; The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome.; Authors - Miao Li
human	WB	Front Genet; Identification of immune signatures in Parkinson's disease based on co-expression networks; Authors - Xiaolin Dong