SERCA2,ATP2A2 Monoclonal antibody

SERCA2,ATP2A2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat and More (3)







Cat no : 67248-1-Ig


ATP2A2, ATP2B, Calcium pump 2, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, SERCA2, SERCA2,ATP2A2, SERCA2b, SR Ca(2+) ATPase 2

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman urothelial carcinoma tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67248-1-Ig targets SERCA2,ATP2A2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERCA2,ATP2A2 fusion protein Ag5052
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated molecular weight 1042 aa, 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC035588
Gene symbol ATP2A2
Gene ID (NCBI) 488
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.


Product Specific Protocols
WB protocol for SERCA2,ATP2A2 antibody 67248-1-IgDownload protocol
IHC protocol for SERCA2,ATP2A2 antibody 67248-1-IgDownload protocol
IF protocol for SERCA2,ATP2A2 antibody 67248-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Physiol

microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.

Authors - Huan Wei

Cell Calcium

Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.

Authors - Yu Chen

Ecotoxicol Environ Saf

Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.

Authors - Leqiang Gao

J Biol Chem

Cryo-EM structure of mouse TRPML2 in lipid nanodiscs

Authors - Xiaojing Song

Clin Transl Med

Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome.

Authors - Jin‐Young Yoon

Int J Biol Macromol

Lysine crotonylation of SERCA2a correlates to cardiac dysfunction and arrhythmia in Sirt1 cardiac-specific knockout mice

Authors - Huan-Xin Chen


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Susmita (Verified Customer) (06-13-2022)

This antibody is very good for WB analysis.

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: PC cell line

Iram (Verified Customer) (03-11-2022)

Very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000 for western
  • Cell Tissue Type: Colon cancer cells