SERCA2,ATP2A2 Monoclonal antibody

SERCA2,ATP2A2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F11A11

Cat no : 67248-1-Ig

Synonyms

ATP2A2, ATP2B, Calcium pump 2, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, SERCA2, SR Ca(2+) ATPase 2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67248-1-Ig targets SERCA2,ATP2A2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERCA2,ATP2A2 fusion protein Ag5052
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated molecular weight 1042 aa, 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC035588
Gene symbol ATP2A2
Gene ID (NCBI) 488
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.

Protocols

Product Specific Protocols
WB protocol for SERCA2,ATP2A2 antibody 67248-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Physiol

microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.

Authors - Huan Wei