SERCA2,ATP2A2 Monoclonal antibody
SERCA2,ATP2A2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, Mouse, Rat and More (3)
WB, IHC, IF, ELISA
Cat no : 67248-1-Ig
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HepG2 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IHC detected in||human urothelial carcinoma tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:3000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67248-1-Ig targets SERCA2,ATP2A2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SERCA2,ATP2A2 fusion protein Ag5052|
|Full Name||ATPase, Ca++ transporting, cardiac muscle, slow twitch 2|
|Calculated molecular weight||1042 aa, 115 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC035588|
|Gene ID (NCBI)||488|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.
|Product Specific Protocols|
|WB protocol for SERCA2,ATP2A2 antibody 67248-1-Ig||Download protocol|
|IHC protocol for SERCA2,ATP2A2 antibody 67248-1-Ig||Download protocol|
|IF protocol for SERCA2,ATP2A2 antibody 67248-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.
Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.
Ecotoxicol Environ Saf
Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.
J Biol Chem
Cryo-EM structure of mouse TRPML2 in lipid nanodiscs
Clin Transl Med
Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susmita (Verified Customer) (06-13-2022)
This antibody is very good for WB analysis.
Iram (Verified Customer) (03-11-2022)
Very good antibody