|Positive WB detected in||HEK-293 cells, HepG2 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:3000-1:20000|
|Sample-dependent, check data in validation data gallery|
67248-1-Ig targets SERCA2,ATP2A2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SERCA2,ATP2A2 fusion protein Ag5052|
|Full Name||ATPase, Ca++ transporting, cardiac muscle, slow twitch 2|
|Calculated molecular weight||1042 aa, 115 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC035588|
|Gene ID (NCBI)||488|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types.