SERF1A Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SERF1A Polyclonal antibody (12725-1-AP)

SERF1A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 12725-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Small EDRK rich factor 1, Small EDRK-rich factor 1

• 

  WB analysis using 12725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12725-1-AP (SERF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 12725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SERF1A antibody (12725-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12725-1-AP targets SERF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3422

  Product name: Recombinant human SERF1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-62 aa of BC021174

  Sequence: MARGNQRELARQKNMKKTQEISKGKRKEDSLTASQRKQRDSEIMQEKQKAANEKKSMQTREK

• Full Name: small EDRK-rich factor 1A (telomeric)
• Calculated Molecular Weight: 62 aa, 7 kDa
• Observed Molecular Weight: 12-15 kDa
• GenBank Accession Number: BC021174
• Gene Symbol: SERF1A
• Gene ID (NCBI): 8293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75920
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small EDRK-rich factor 1 (SERF1A) is also named SMA modifier 1 (SMAM1). SERF1a is part of a 500 kb inverted duplication on chromosome 5q13 and highly related to spinal muscular atrophy. Human SERF1a protein has two isoforms. The short form contains 62 residues, and the long form has 110 amino acids. They share a highly conserved N-terminal region, and they are enriched in lysine and arginine (PMID: 37479809). SERF1a was found to promote amyloid formation in vitro on several amyloid proteins, including α-synuclein, huntingtin, Aβ, and prion proteins (PMID: 22854022). SERF1a has been reported as an RNA-binding protein capable of mediating the functional integration of RNA in the nucleolus (PMID: 31794729). This target may exhibit cross-reaction with SERF2.