SERINC1 Polyclonal antibody

SERINC1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20265-1-AP

Synonyms

KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2

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Validation Data Gallery

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  WB analysis of HeLa using 20265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20265-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20265-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20265-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells
• Positive IHC detected in: human liver cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20265-1-AP targets SERINC1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine incorporator 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_020755
• Gene Symbol: SERINC1
• Gene ID (NCBI): 57515
• RRID: AB_10693613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERINC1, also named as KIAA1253, TDE1L and TDE2, belongs to the TDE1 family. SERINC1 enhances the incorporation of serine into phosphatidylserine and sphingolipids. The antibody is specific to SERINC1.