Filter: AllWBIHCIF-PIF/ICC

WB analysis using 60113-1-Ig (same clone as 60113-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60113-1-Ig (SERPINA10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.
IF Staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.
IF Staining of human liver using 60113-1-Ig (same clone as 60113-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 60113-1-Ig (same clone as 60113-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60113-1-PBS in a different storage buffer formulation.

SERPINA10 Monoclonal antibody, PBS Only

SERPINA10 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 60113-1-PBS

Clone No.5B3C9

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

5B3C9, Protein Z-dependent protease inhibitor, PZ-dependent protease inhibitor, PZI, Serpin A10

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60113-1-PBS targets SERPINA10 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity	human, rat, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2420 Product name: Recombinant human SERPINA10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 137-444 aa of BC022261 Sequence: KPGLLPSLFKGLRETLSRNLELGLTQGSFAFIHKDFDVKETFFNLSKRYFDTECVPMNFRNASQAKRLMNHYINKETRGKIPKLFDEINPETKLILVDYILFKGKWLTPFDPVFTEVDTFHLDKYKTIKVPMMYGAGKFASTFDKNFRCHVLKLPYQGNATMLVVLMEKMGDHLALEDYLTTDLVETWLRNMKTRNMEVFFPKFKLDQKYEMHELLRQMGIRRIFSPFADLSELSATGRNLQVSRVLQRTVIEVDERGTEAVAGILSEITAYSMPPVIKVDRPFHFMIYEETSGMLLFLGRVVNPTLL Predict reactive species
Full Name	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Calculated Molecular Weight	444 aa, 51 kDa
Observed Molecular Weight	51 kDa
GenBank Accession Number	BC022261
Gene Symbol	SERPINA10
Gene ID (NCBI)	51156
RRID	AB_2301526
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UK55
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.