SERPINB10 Polyclonal antibody

• 

  WB analysis using 21719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21719-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 21719-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21719-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21719-1-AP (SERPINB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 21719-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21719-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 21719-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 21719-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21719-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINB10 antibody 21719-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

SERPINB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21719-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10

---

Tested Applications

• Positive WB detected in: A431 cells, HL-60 cells, Sp2/0 cells, human saliva
• Positive IHC detected in: human tonsillitis tissue, human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

21719-1-AP targets SERPINB10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16372

  Product name: Recombinant human SERPINB10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-307 aa of BC096217

  Sequence: MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDPESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQPVNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRINETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQLHLPKFKLEDSYDLKSTLS

• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 10
• Calculated Molecular Weight: 397 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC096217
• Gene Symbol: SERPINB10
• Gene ID (NCBI): 5273
• RRID: AB_10734592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48595
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	Am J Physiol Lung Cell Mol Physiol; Epithelial SERPINB10, a novel marker of airway eosinophilia in asthma, contributes to allergic airway inflammation.; Authors - Yuqing Mo ; KD Validated