SERPINB6 Polyclonal antibody

• Featured Product
• KD/KO Validated

SERPINB6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14962-1-AP

Synonyms

PI6, PTI, Serpin B6, PI-6, PI 6

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Validation Data Gallery

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  WB analysis using 14962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14962-1-AP

  WB result of SERPINB6 antibody (14962-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SERPINB6 transfected HeLa cells.

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  WB analysis of mouse skeletal muscle using 14962-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14962-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14962-1-AP (SERPINB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14962-1-AP

  IP result of anti-SERPINB6 (IP:14962-1-AP, 4ug; Detection:14962-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human skeletal muscle using 14962-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14962-1-AP (SERPINB6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 14962-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14962-1-AP (SERPINB6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, mouse skeletal muscle tissue, HepG2 cells, MCF-7 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:18000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

14962-1-AP targets SERPINB6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINB6 fusion protein Ag6824
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 6
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001394
• Gene Symbol: SERPINB6
• Gene ID (NCBI): 5269
• RRID: AB_2186456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Tissue Res; Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouse submandibular gland.; Authors - Hiroki Nakata
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
mouse	WB	PLoS One; New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.; Authors - Flora Cozzolino
human	WB,IHC	Cell Mol Gastroenterol Hepatol; Single-cell transcriptomics reveals early molecular and immune alterations underlying the serrated neoplasia pathway toward colorectal cancer; Authors - Yu-Jie Zhou